Biotin-16-UTP

Biotin-16-UTP

Author: ApexBio

purity:

≥90% by AX-HPLC

shortsummary:

A biotin-labeled nucleotide analog, used to form labeled RNA

Calcium Phosphate Cell Transfection

Synonyms:

Biotin-16-Aminoallyluridine-5'-Triphosphate, Biotin-16-UTP

phosphatase inhibitor cocktail 2

Formula:

C32H52N7O19P3S (free acid)

M.Wt:

963.8 (free acid)

Chemical Name:

lithium (E)-(5-(2,4-dioxo-5-(3-(4-(6-(5-(2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)butanamido)prop-1-en-1-yl)-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl triphosphate

SMILES:

O=C(C(/C=C/CNC(CCCNC(CCCCCNC(CCCCC1C(NC(N2)=O)C2CS1)=O)=O)=O)=CN3C4C(O)C(O)C(COP([O-])(OP([O-])(OP([O-])([O-])=O)=O)=O)O4)NC3=O.[Li+].[Li+].[Li+].[Li+]

Storage:

Store at -20°C or below

Physical Appearance:

Solution

DeliveryTime:

In stock

Con A

ShippingType:

Dry ice

description:

Biotin-16-UTP is a biotin-labeled nucleotide analog, which can be incorporated via a variety of RNA polymerases, including SP6, T3 and T7 RNA polymerases to form labeled RNA. Since T7 RNA polymerase does not significantly discriminate between the natural and the biotinylated nucleotide, the number of biotinylated positions per RNA transcript can be controlled by adjusting the ratio of the biotinylated nucleoside triphosphate (NTP) to the naturally occurring NTP in the transcription reaction. The biotin-labeled RNA can then be detected by a reporter molecule attached to streptavidin, avidin, or anit-biotin antibody. Biotinylated RNA probes are useful as a nonradioactive label in a number of applications including chromosome mapping, in situ hybridization, as well as microarray hybridization.

related websites:

APExBIO Technology LLC is a premier provider of Small Molecule Inhibitors/Activators, Compound Libraries, Peptides, Assay Kits, Fluorescent Labels, Enzymes, Modified Nucleotides, mRNA synthesis and various tools for Molecular Biology. We carry a broad product line in over 7302 different research areas such as cancer, immunology, neurosciences, apoptosis and epigenetics etc. Based in USA (Houston, Texas), we have been serving the needs of customers across the world.